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Fig. 3 Increased Hedgehog signaling expands cardiomyocyte numbers. (A-F) Dorsal views, anterior towards the top. Scale bars: 100 μm. (A-C) nkx2.5 expression at the seven-somite stage is subtly expanded in shh-injected and ptc1;ptc2 double mutant embryos. The observed extent of anterior-posterior enlargement was variable. (D-F) cmlc2 expression at the 18-somite stage is expanded in shh-injected and ptc1;ptc2 double mutant embryos. The degree of expansion was variable and frequently coupled with disorganized morphology of migrating cardiomyocyte populations, as in E.
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