ZFIN Image: Clément et al., 2008, Fig. 3

Image

Figure Caption

Fig. 3 pic^-/- encodes a PAPS transporter.

Genetic map of pic locus (A). Note that z1534 has not been assigned a genetic position as it was found on the RH map. Allele sequencing identifies pic^to216z as a premature stop codon in exon 3 and pic^to14mx as a deletion of exon 3 (B). Zebrafish PAPST1 clusters with human and Drosophila PAPST1 in a dendrogram (C). Expression of wild-type PAPST1 in a single notochord cell rescues synthesis of KS in pic^-/- larvae (D–E). Close-up of single cell secreting KS in brown (arrow in E), and the same cell counterstained for the rescue construct reporter in purple (arrow in F). The dendrogram was made using Clustal W and Treeview68k. Accession numbers: AB107958, NP_648954, AB106538, NP_057032, NP_991198, NP_001035084, NM_005827. Zebrafish and human PAPST1 share 70% amino acid identity. Panel D scale bar = 100μM. Panel E scale bar = 50μM.

Acknowledgments

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