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Fig. 2 Catecholamine cell groups in the peripheral nervous system of zebrafish embryos. Whole-mount embryos or transverse sections labeled with anti-TH antibody or antisense TH RNA probe. (A) TH-positive cells (indicated with black arrowheads) adjacent to the rostral hindbrain of 22S embryo—dorsal view. Rhombomeres r3 and r5 are labeled by Krox-20. (B–D) Ventral (B and D) and lateral (C) views of THir cells in 50-h (B and C) and 72-h (D) embryos. Arrowheads indicate THir cells posterior to the eyes. Rostral is up in D. (E) Transverse section through the pharyngeal arches of a 96-h fry showing THir cells located between the sternohyoid muscles of arch 3. (F) Prim-25 (36 h) embryo showing THir cells located at the interface between primordial arches 2 and 3, rostral to the gill arches (labeled in black with zn-8 antibody). (G) Putative sympathetic THir cells ventral and lateral to the rostral notochord of a 96-h fry. (H) Sympathetic (sym) THir neurons in a 96-h fry. Abbreviations: a, aorta; aac, arch-associated CA cells; cb, ceratobranchial; fp, floor plate; g1, 2, 3, and 4, gills 1, 2, 3, and 4; H, hyoid; M, mandibular; m, muscle; n, notochord; P2 and P3, pharyngeal arches 2 and 3; sh, sternohyoid; sym, sympathetic neurons; tr, trigeminal ganglia; tv, transverse ventral muscle; v, blood vessel. Scale bar: 80 μm (A), 100 μm (B, C, F), 50 μm (D, H), and 25 μm (E, G).
Reprinted from Developmental Biology, 208, Guo, S., Wilson, S.W., Cooke, S., Chitnis, A.B., Driever, W., and Rosenthal, A., Mutations in the zebrafish unmask shared regulatory pathways controlling the development of catecholaminergic neurons, 473-487, Copyright (1999) with permission from Elsevier. Full text @ Dev. Biol.