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Fig. S2
miR-9 expression in the 3dpf zebrafish brain.
miR-9 is expressed in both periventricular and adjacent cells of proliferative zones and differentiating cells arising from these domains. miR-9 expressing cells are widespread in all brain subdivisions (fore-, mid- and hind-brain) although some areas such as the larval epithalamus and the hypothalamic lateral torus are devoid of expression (Table A). In addition to the brain, miR-9 is expressed in the inner retinal nuclear layer and ciliary marginal zone. miR-9 expression is highly conserved between larval and adult brain and we find only minor differences at the regional level (Table A,F). miR-9 is expressed in a few adult habenular and hypothalamic lateral torus cells, areas devoid of larval expression.
A. transverse section through the larval rostral telencephalon at the level of the olfactory epithelium (OE) showing miR-9 expressing cells in the olfactory bulb (OB). Expression is absent in cells immediately ventral to the bulb.
B. transverse section through the larval telencephalon showing miR-9 expressing cells in the ventral (Sv) and dorsal (Sd) subpallium, pallium (P) and olfactory bulb (OB).
C. transverse section through the larval caudal telencephalon and epithalamus showing miR-9 expressing cells in the ventral (Sv) and dorsal (Sd) subpallium, pallium (P) and migrated telencephalic area (M4). Expression is absent from the habenulae and epiphysis.
D. transverse section through the larval diencephalon and rostral optic tectum showing miR-9 expressing cells in the preoptic area (Po), eminentia thalami (ET), migrated eminentia thalami (M3), ventral thalamus (VT), dorsal thalamus (DT), zona limitans intrathalamica (ZLI) and the optic tectum (TeO).
E. transverse section through the larval diencephalon and optic tectum showing miR-9 expressing cells in the preoptic area (Po), ventral thalamus (VT), dorsal thalamus (DT), periventricular (Pr) and migrated (M1) pretectal areas, optic tectum (TeO), retinal inner nuclear layer (INL) and ciliary marginal zone (arrowhead).
F. transverse section through the larval diencephalon and optic tectum showing miR-9 expressing cells in the rostral hypothalamus (Hr), periventricular posterior tuberculum (PT), ventral thalamus (VT), dorsal thalamus (DT), periventricular (Pr) and migrated (M1) pretectal areas, proliferative (m) and periventricular gray (pgz) zones of the optic tectum (TeO).
G. transverse section through the larval diencephalon and optic tectum showing miR-9 expressing cells in the rostral hypothalamus (Hr), ventral (PTv), dorsal (PTd) periventricular and migrated (M2) posterior tuberculum, dorsal thalamus (DT), periventricular pretectal area (Pr) and periventricular gray (pgz) zone of the optic tectum (TeO).
H. transverse section through the larval diencephalon and midbrain showing miR-9 expressing cells in the rostral hypothalamus (Hr), ventral (PTv), dorsal (PTd) periventricular and migrated (M2) posterior tuberculum, tegmentum (T), proliferative (m) and periventricular gray (pgz) zones of the optic tectum (TeO).
I. transverse section through the larval hypothalamus and midbrain showing miR-9 expressing cells in the intermediate hypothalamus (Hi, around the lateral ventricular recess, lr), ventral periventricular (PTv) and migrated (M2) posterior tuberculum, tegmental area at the level of the nucleus of the medial longitudinal fascicle (N), semicircular torus (TS) and periventricular gray zone (pgz) of the optic tectum (TeO).
J. transverse section through the larval hypothalamus and midbrain showing miR-9 expressing cells in the intermediate hypothalamus (Hi) at the level of the lateral hypothalamic ventricular recess (lr), tegmental area (T), occulomotor nucleus (NIII), semicircular torus (TS) and periventricular gray zone (pgz) of the optic tectum (TeO). The arrowhead points at the oculomotor nerve (III).
K. transverse section through the larval caudal hypothalamus and midbrain showing miR-9 expressing cells at the level of the lateral hypothalamic ventricular recess (lr), caudal hypothalamus (Hc), tegmental area (T), semicircular torus (TS), periventricular gray zone (pgz) of the optic tectum (TeO) and cerebellar valvula (Va).
L. transverse section through the larval caudal hypothalamus, midbrain and isthmus showing miR-9 expressing cells in the interpeduncular nucleus (NIn), isthmic area (Is), semicircular torus (TS), cerebellar plate (CeP) and optic tectum (TeO). M. transverse section through the larval hindbrain at the level of the octaval ganglion (OG) showing miR-9 expressing cells in the medulla oblongata (MO) and cerebellar plate (CeP).
N. transverse section through the larval hindbrain at the level of the otic capsule (caudal to section M) showing miR-9 expressing cells in the medulla oblongata (MO), presumptive octaval area (OA?) and rhombic lip (RL).
O. transverse section through the larval hindbrain at the level of the otic capsule (caudal to section N) showing miR-9 expressing cells in the medulla oblongata (MO), presumptive octaval area (OA?) and rhombic lip (RL).