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Fig. S6 Dystrophin expression is normal in psm7m/m embryos. Immunostaining of dystrophin (red) in muscle myotomes of 3.5 dpf psm7m/m zebrafish embryos (B) is similar to that in wild-type embryos (A), indicating that dystrophin-related muscle attachment is not defective in this mutant. Staining was performed as described by Bassett et al. [67] with the exception that the secondary antibody used was Alexa Fluor 594-conjugated anti-mouse IgG at a 1:1000 dilution.
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