ZFIN Image: Goessling et al., 2008, Fig. S6

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Figure Caption

Fig. S6 The effects of wnt activation on mature endodermal organs persist into the larval stage. (A–I) Confocal microscopy and FACS analysis of live zebrafish larvae at 144 hpf (6 dpf). (A–C) APC^+/^-; lfabp:GFP larvae maintain increased liver size; this is confirmed by FACS analysis. (D–F) The pancreas of APC^+/^-; ptf1α:GFP larvae remains smaller than that of wild-type siblings. (G–H) The effect on the intestine as indicated in ifabp:GFP reporter fish parallels that of the liver. (J) Induction of wnt8 after liver differentiation at 48 hpf increased the number of LFABP:GFP+ hepatocytes at 72 (wild-type 1.25 ± 0.12% vs. wnt8 2.42 ± 0.43%, n = 10, p < 0.0005) and 120 hpf (wild-type 2.17 ± 0.19% vs. wnt8 5.21 ± 0.37%, n = 10, p < 0.0005).

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Acknowledgments

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Reprinted from Developmental Biology, 320(1), Goessling, W., North, T.E., Lord, A.M., Ceol, C., Lee, S., Weidinger, G., Bourque, C., Strijbosch, R., Haramis, A.P., Puder, M., Clevers, H., Moon, R.T., and Zon, L.I., APC mutant zebrafish uncover a changing temporal requirement for wnt signaling in liver development, 161-174, Copyright (2008) with permission from Elsevier. Full text @ Dev. Biol.