ZFIN Image: Heanue et al., 2008, Fig. S1

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Figure Caption

Fig. S1 Sequence specificity for ret and ret51Sp MOs is assessed using control morpholinos containing five mismatched nucleotides along their length (mismatch MOs). Following injection of ret mismatch MO (B) or ret51Sp mismatch MO (D), fish are analyzed for the presence of Hu-expressing enteric neurons at 4dpf and compared to uninjected control fish (A, C). No difference in number or distribution of Hu-expressing enteric neurons is detected in these fish (A–D). This finding provides important control for the sequence specificity of ret and ret51Sp MOs used in Figs. 2 and 3, respectively. Critically, this result helps to demonstrate that the retMO effect on ENS number shown in Fig. 2 is sequence specific. Arrows indicate distal most enteric neurons. Images for comparison are photographed at the same magnification.

Acknowledgments

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Reprinted from Mechanisms of Development, 125(8), Heanue, T.A., and Pachnis, V., Ret isoform function and marker gene expression in the enteric nervous system is conserved across diverse vertebrate species, 687-699, Copyright (2008) with permission from Elsevier. Full text @ Mech. Dev.