ZFIN Image: Lee et al., 2008, Fig. 5

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Figure Caption

Fig. 5 The proximal/distal axis of the optic vesicle is disrupted in blowout. pax2a marks the proximal OV (optic stalk) in wild-type embryos at 15 hpf (A), 18 hpf (B), 24 hpf (G) and 36 hpf (H), while it is absent at 48 hpf (I) owing to the degeneration of the stalk by this time. Expression in blw mutants is observed in a broader domain within the optic stalk region as early as 15 hpf (B) and this expansion is maintained at 18 hpf (E), 24 hpf (J) and 36 hpf (K, arrow). Expression is not extinguished at 48 hpf and can be observed to extend into the retina (L, arrow). Conversely, pax6 marks the distal optic vesicle (retina and RPE) at 18 hpf (C), and expression is substantially contracted within distal optic vesicle of blw mutants (F) in a pattern complementary to the expanded domain of pax2a.

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Acknowledgments

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Reprinted from Developmental Biology, 319(1), Lee, J., Willer, J.R., Willer, G.B., Smith, K., Gregg, R.G., and Gross, J.M., Zebrafish blowout provides genetic evidence for Patched1-mediated negative regulation of Hedgehog signaling within the proximal optic vesicle of the vertebrate eye, 10-22, Copyright (2008) with permission from Elsevier. Full text @ Dev. Biol.