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Fig. 8 Foxa2 protein binds to the M1 motif of ctgf up1 and sox9a E1 CRMs. (A) Recombinant Foxa2 binds specifically to M1 of the ctgf up1 CRM. Radioactively labeled oligonucleotide containing ctgf up1 M1 (lane 1) was incubated with Foxa2 protein without competitor oligonucleotide (lane 2), with a 50-fold molar excess of cold ctgf up1M1 oligonucleotide (lane 3), or with an oligonucleotide that contained a mutated M1 sequence (lane 4). Cold M1 competitor abolished the shifted band (lane 3), whereas mutated oligonucleotide did not (lane 4). Radioactively labeled mutated ctgf up1 M1 probe alone (lane 5) or in the presence of Foxa2 protein (lane 6) does not show retarded DNA protein complexes. (B) FoxA2 binds specifically to the sox9a E1M1. Radioactively labeled oligonucleotide containing sox9a E1M1 (lane 1) was incubated with FoxA2 protein without competitor oligonucleotide (lane 2), with a 50-fold molar excess of cold sox9a E1 M1 oligonucleotide (lane 3), or with an oligonucleotide that contained a mutated sox9a E1 M1 sequence (lane 4). Cold M1 competitor abolished the shifted band (lane 3), whereas mutated oligonucleotide did not (lane 4). Radioactively labeled mutated sox9a E1 M1 probe alone (lane 5) or in the presence of Foxa2 protein (lane 6) does not show retarded DNA protein complexes.
Reprinted from Developmental Biology, 318(2), Rastegar, S., Hess, I., Dickmeis, T., Nicod, J.C., Ertzer, R., Hadzhiev, Y., Thies, W.G., Scherer, G., and Strähle, U., The words of the regulatory code are arranged in a variable manner in highly conserved enhancers, 366-377, Copyright (2008) with permission from Elsevier. Full text @ Dev. Biol.