ZFIN Image: Rastegar et al., 2008, Fig. 5

Image

Figure Caption

Fig. 5 Linker scanning analysis of the sox9a E1 enhancer identifies a subregion as necessary for notochord expression. (A) Reporter constructs designed to test the activity of the E1 enhancer. A 348 bp fragment containing a 135 base pair (bp) conserved core (E1 core) of the sox9a E1 enhancer was cloned downstream of the 2.4 kb shh promoter and gfp reporter gene (- 2.4shh:GFP). Clusters of 20 bp transition mutations are represented by black boxes. Numbers represent the positions relative to the sox9a ATG. (B-M) Lateral view of 26 h old zebrafish embryos, injected with the constructs RC0 to RC11. Expression in the notochord (n) is abolished in embryos injected with the constructs RC6 to RC9. Remaining anterior floor plate (fp) expression of - 2.4shh:GFP serves as an injection control. Anterior to the left and dorsal up. Scale bar = 250 μm.

Acknowledgments

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Reprinted from Developmental Biology, 318(2), Rastegar, S., Hess, I., Dickmeis, T., Nicod, J.C., Ertzer, R., Hadzhiev, Y., Thies, W.G., Scherer, G., and Strähle, U., The words of the regulatory code are arranged in a variable manner in highly conserved enhancers, 366-377, Copyright (2008) with permission from Elsevier. Full text @ Dev. Biol.