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Fig. 4 GFP–Ilk and paxillin protein localizations in itgα7 and lama4 morphant embryos. (A–C) Injection of gfp–ilk mRNA in wt embryos results in GFP–Ilk protein localization to the MTJ at the somite boundaries in 24 hpf embryos (A). Reduced GFP–Ilk protein localization in embryos co-injected with an itgα7–ATG MO (B) or lama4–ATG MO (C). (D–F) Anti-paxillin antibody staining on wt embryos (D). Reduced paxillin localization to the MTJ at the somite boundary in itgα7–ATG MO (E) or lama4–ATG MO (F) injected embryos at 24 hpf.
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Reprinted from Developmental Biology, 318(1), Postel, R., Vakeel, P., Topczewski, J., Knöll, R., and Bakkers, J., Zebrafish integrin-linked kinase is required in skeletal muscles for strengthening the integrin-ECM adhesion complex, 92-101, Copyright (2008) with permission from Elsevier. Full text @ Dev. Biol.