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Fig. S1

ID: ZDB-IMAGE-080516-41
Source: Figures for Qu et al., 2008

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Figure Caption

Fig. S1 In vivo validation of NDRG4 MO1 (knockdown by translation-blocking). (A) Schematic of the strategy to target the translational start site with NDRG4 MO1. (B) Schematic map of a transgenic construct in which the cmlc2 promoter is upstream of an NDRG4-EYFP fusion DNA. H19 insulators in the pWhere plasmid (InvivoGen) flanked the cmlc2-NDRG4-EYFP DNA fragment, which includes the binding region of NDRG4,/em> MO1. (C) After Co-injection of the linearized DNA with 6.4 ng of control MO (a) or NDRG4 MO1 (b) into wildtype embryo at one-cell stage, green fluorescent signals due to NDRG4-EYFP expression in cardiomyocytes were observed in 7.9% (control MO) or 0% (NDRG4 MO1) of injected embryos at 48 hpf. Arrow indicates the heart region.

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Reprinted from Developmental Biology, 317(2), Qu, X., Jia, H., Garrity, D.M., Tompkins, K., Batts, L., Appel, B., Zhong, T.P., and Baldwin, H.S., ndrg4 is required for normal myocyte proliferation during early cardiac development in zebrafish, 486-496, Copyright (2008) with permission from Elsevier. Full text @ Dev. Biol.