ZFIN Image: Shin et al., 2008, Fig. S3

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Figure Caption

Fig. S3 Analysis of apoptosis in med12^s435 mutant embryos. (A, A′) Confocal projections of Prox1 (blue) and Actin (red) expression in wild-type and med12^s435 mutants in the Tg(gutGFP)^s854 line (green) at 34 hpf. White dashed lines surround the endodermal region. (C–F) Confocal projections of wild-type and med12^s435 mutants in the Tg(gutGFP)^s854 line (green) at 34 (C, D) and 48 (E, F) hpf in conjunction with anti-Prox1 (blue) antibody and TUNEL (red) staining. White dashed lines indicate the midline. In med12^s435 mutants, Prox1 expression is mostly missing at 34 hpf but present at 48 hpf. Little if any TUNEL staining was observed in the endodermal tissues at these stages. (G–H) Confocal projections of TUNEL staining in the brain region of wild-type and med12^s435 mutants. Asterisks indicate the eyes and arrowheads point to TUNEL-positive cells. Transverse sections, dorsal to the top.

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Acknowledgments

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Reprinted from Developmental Biology, 317(2), Shin, C.H., Chung, W.S., Hong, S.K., Ober, E.A., Verkade, H., Field, H.A., Huisken, J., and Stainier, D.Y., Multiple roles for Med12 in vertebrate endoderm development, 467-479, Copyright (2008) with permission from Elsevier. Full text @ Dev. Biol.