ZFIN Image: Shin et al., 2008, Fig. 5

Image

Figure Caption

Fig. 5 Med12 regulates her5 expression in endodermal cells during gastrulation and somitogenesis. (A, A′) In situ hybridization at 90% epiboly shows specific reduction of her5 expression in endodermal cells in med12^s432 mutants compared to wild-type siblings. The V shaped stripe represents the domain of her5 expression in the midbrain–hindbrain boundary. (B–D′) Injection of med12 MO into the Tg(-0.7her5:EGFP)^ne2067 line caused decreased GFP expression. Note that the Tg(-0.7her5:EGFP)^ne2067 line shows GFP expression in the pharyngeal endoderm as well as the organ-forming region during late somitogenesis stages (C, D). White bracket in C–D′ indicates pharyngeal region. Exposure time for imaging B, B′ was 300 ms; C, C′ was 250 ms; D, D′ was 250 ms. Dorsal views, anterior to the top.

Figure Data

Expression / Labeling details

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image.

Reprinted from Developmental Biology, 317(2), Shin, C.H., Chung, W.S., Hong, S.K., Ober, E.A., Verkade, H., Field, H.A., Huisken, J., and Stainier, D.Y., Multiple roles for Med12 in vertebrate endoderm development, 467-479, Copyright (2008) with permission from Elsevier. Full text @ Dev. Biol.