ZFIN Image: Willot et al., 2002, Fig. 11

Image

Figure Caption

Fig. 11 admp antagonizes organizer formation and maintenance. (A, B) Injection of mouse Δ-alk3 RNA at the 16-cell stage into one marginal blastomere leads to induction of a partial secondary axis. Arrowheads, otic vesicles. (A) Bright-field and epifluorescence images are shown side by side. The use of NLS-GFP as a lineage tracer reveals the induced axis. (B, C) In situ hybridization with pax6, her5, and myoD probes in dark blue, hgg1 in red. (C) admp-morpholino injection at the one-cell stage followed by mouse Δ-alk3 RNA injection at the 16-cell stage leads to induction of a partial secondary axis with forebrain structures. The forebrain stained with pax6 (black arrow) and midbrain–hindbrain boundary stained with her5 (red arrow) are indicated.

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image.

Reprinted from Developmental Biology, 241(1), Willot, V., Mathieu, J., Lu, Y., Schmid, B., Sidi, S., Yan, Y.-L., Postlethwait, J.H., Mullins, M., Rosa, F., and Peyriéras, N., Cooperative action of ADMP- and BMP-mediated pathways in regulating cell fates in the zebrafish gastrula, 59-78, Copyright (2002) with permission from Elsevier. Full text @ Dev. Biol.