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Fig. 5 The response of Xenopus animal caps to microinjection of pitx2 mRNA partially mimics the response to Xnr-1. Embryos at the eight-cell stage were microinjected at the animal pole in each blastomere with 25, 75, or 225 pg of pitx2c, pitx2b, or Xnr-1 mRNA. Animal caps were removed at stage 9 and analyzed at stage 11 by RT-PCR for expression of the marker genes indicated. 2RT, control without reverse transcription. pitx2 dose-dependently induces goosecoid, sox17β, cerberus, and antivin but not Xbra or chordin. These results are representative of four independent experiments.
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Reprinted from Developmental Biology, 229(2), Faucourt, M., Houliston, E., Besnardeau, L., Kimelman, D., and Lepage, T., The pitx2 homeobox protein is required early for endoderm formation and nodal signaling, 287-306, Copyright (2001) with permission from Elsevier. Full text @ Dev. Biol.