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Fig. 6 The fby mutation is a premature stop codon in the tbx2b gene. (A) Diagram of zebrafish chromosome 15, showing the location of the fby mutation relative to nearby SSLP markers. (B) In fbyc144 mutants, the third position of codon 245 in the tbx2b gene was transverted from T to A, generating a stop codon within the T-box sequence. (C,D) Frontal views of 4-dpf larvae. Compared with uninjected foxd3:gfp controls (C), injection of an antisense morpholino against the start codon of tbx2b (ATG MO) resulted in a similar phenotype to the fby mutation (D). Scale bar: 25 μm.
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