ZFIN Image: Kreiling et al., 2008, Fig. 5

Image

Figure Caption

Fig. 5 Ca²⁺-sensitive gene expression in the dorsal forerunner cells. (A–F) Syto11 labeling in the dorsal forerunner cells (DFC) at 7 hpf. The dorsal forerunner cells are present in the same location in the DMSO-treated control embryos (A–C), as they are in the thapsigargin-treated embryos (D–F). (G–L) ntl and lrdr RNA in situ hybridizations. DMSO-treated control embryos express ntl in the margin, notochord, and dorsal forerunner cells at 7 hpf (G and H) and express lrdr in the dorsal forerunner cells at 8 hpf (I). Thapsigargin treatment resulted in a strong down-regulation of ntl expression in the dorsal forerunner cells (J and K) and led to a complete inhibition of lrdr expression (L). Scale bar = 50 μm.

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Expression / Labeling details

Acknowledgments

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Reprinted from Mechanisms of Development, 125(5-6), Kreiling, J.A., Balantac, Z.L., Crawford, A.R., Ren, Y., Toure, J., Zchut, S., Kochilas, L., and Creton, R., Suppression of the endoplasmic reticulum calcium pump during zebrafish gastrulation affects left-right asymmetry of the heart and brain, 396-410, Copyright (2008) with permission from Elsevier. Full text @ Mech. Dev.