Fig. 3

Fig. 3 Expression of zebrafish trpC6 by whole mount in situ hybridization and histological analysis. Expression of trpC6 mRNA is ubiquitous at six somites (A, inset) and in all stages tested up to and including 24 hpf (A). (B) At 48 hpf, expression becomes restricted to the head, pectoral fins (black arrowhead), the area of the gut (white arrows) extending to the posterior end (black arrow). Dorsal view of expression in fins at 48 hpf (B, inset). This pattern of trpC6 expression persists to 72 hpf (C), where expression in the most posterior region of the gut (black arrow and inset) remains high while more proximal regions of the gut show diminished expression. Histological examination reveals that trpC6 expression in the pectoral fins is restricted to the dorsal surface (D, black arrowheads). Sectioning of the trunk of 72 hpf embryos shows that trpC6 mRNA is expressed in cells lining the dorsal aorta (E, black arrowheads). A closer examination of the gut reveals that trpC6 is highly expressed in cells that surround and encapsulate the gut (F, black arrows). (G) Sections through the glomerulus of a 3 dpf larva (G, dashed black circle) demonstrate that trpC6 RNA is not detectably expressed podocytes, whereas cells encapsulating the anterior gut are positive for trpC6 (G, white arrowheads). Later stage, 5 dpf (H) and adult glomeruli (I) do not display specific labeling for trpC6. nc, notochord; gl, glomerulus; t, tubules.