ZFIN Image: Rastegar et al., 2002, Fig. 3

Image

Figure Caption

Fig. 3 Stable expression of the –1.1netrin1:gfp/FPE transgene. (A) Expression of the transgene in floor plate (f) and hypochord (h) of a 32-hpf embryo. (B, C) Whole-mount in situ hybridisation of transgenic embryos with gfp antisense RNA at the 32-hpf (B) and one-somite stages (C). (D, E) Comparison of expression of netrin1 (D) and gfp (E) mRNA in the head of 24-hpf transgenic embryos. Note that, unlike netrin1 (D), the transgene (E) is not expressed in the ventral forebrain of embryos. (A, B, D, E) Lateral views, anterior left and dorsal up. (C) Dorsal view, anterior up. Abbreviations: f, floor plate; h, hypochord. Scale bar represents 50 μm (A, B, D, E) and 100 μm (C).

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image.

Reprinted from Developmental Biology, 252(1), Rastegar, S., Albert, S., Le Roux, I., Fischer, N., Blader, P., Müller, F., and Strähle, U., A floor plate enhancer of the zebrafish netrin1 gene requires Cyclops (Nodal) signalling and the winged helix transcription factor FoxA2, 1-14, Copyright (2002) with permission from Elsevier. Full text @ Dev. Biol.