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Fig. S4 pcdh18 expression in the branchial arches. Orange numbers indicate embryonic or larval age post-fertilization. (A,B) Ventral views of embryos at 72 hpf and 5 dpf, respectively, with the yolk removed. Rostral to the left, left to the top. Dotted lines outline the jaw and branchial arches. Closed arrowheads identify the arches. (A) The 5th arch where the pharyngeal tooth would attach has diffuse but no tooth-specific pcdh18 staining at 72 hpf. (B) A pharyngeal tooth (closed arrow) appeared by 5 dpf when pcdh18 expression had diminished from the embryo. (C) A coronary cryostat section of a stained 72 hpf embryo cut at the level of the arches (the 4th arch is somewhat off the level of the particular section). The cells expressing pcdh18 (open arrowheads) surrounds branchial cartilages (open arrows). Abbreviations: 1-5, 1st to 5th branchial arches; Ey, eyes; Es, esophagial lumen, J, jaw arches.