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Fig. 3 Morpholinos directed against tmie decrease levels of normal transcripts. A: Injection of an anti-tmie morpholino (MOtmie1) directed against the splice donor site of exon 3 resulted in a decreased level of intact transcripts and a substantial increase in shorter tmie transcripts that lacked exon 2 (arrow) at 36 hpf. Very little of the shorter transcript was detected at 36 hpf in uninjected embryos (wt) or in embryos injected with a control morpholino (MOcon). B: Injection of an anti-tmie morpholino (MOtmie2) directed against the splice donor site of exon 2 resulted in decreased levels of intact tmie transcripts at 36 hpf (products amplified from two independent RNA preparations are shown), relative to those present in embryos injected with a control morpholino. Levels of Gapdh transcripts are shown in the bottom panel. Size standards are indicated in kb.