Fig. 3

Fig. 3 The tjp3/zo-3 morpholino causes the loss of Tjp3/Zo-3 protein expression. Immunofluorescence of whole mount zebrafish at 1 dpf (A-D), 2 dpf (E-F), and cryosections at 6 dpf (G-H) stained with anti-Tjp3/Zo-3. Tjp3/Zo-3 protein is lost in morpholino-injected embryos from 1 dpf up to 6 dpf. The morphant phenotype correlates to the loss of Tjp3/Zo-3 since embryos selected for the lack of an apparent phenotype still express Tjp3/Zo-3 protein (data not shown). At 1 dpf there is a loss of Tjp3/Zo-3 labeling in the olfactory placodes (A, B, arrows), otic placodes (A, B arrowheads), and skin (C-F arrowheads). At later stages, Tjp3/Zo-3 protein is absent from the apical membranes of the pronephric ducts (E-H, arrows), and intestine (G, H, arrowheads).