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Fig. S2 A tcf7 morpholino effectively blocks protein translation but does not affect early embryogenesis. (A) Western blot analysis of Tcf7 protein levels indicate reduction of 44 kDa and 46 kDa Tcf7 isoforms in tcf7 morphants. (B) Overall morphology of 24 hpf tcf7 morphants is unaffected. (C–D) Expression of sox3, a neural plate marker, is unaffected at bud stage in tcf7 morphants. Dashed lines outline the neural plate. Scale bars: B = 200 μm; C–D = 100 μm.
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Reprinted from Developmental Biology, 313(1), Bonner, J., Gribble, S.L., Veien, E.S., Nikolaus, O.B., Weidinger, G., and Dorsky, R.I., Proliferation and patterning are mediated independently in the dorsal spinal cord downstream of canonical Wnt signaling, 398-407, Copyright (2008) with permission from Elsevier. Full text @ Dev. Biol.