Fig. 3 Comparison of vmat2 and th expression patterns. (A–H) Whole-mount in situ hybridization detecting vmat2 (A–D) and th (E–H) expression in 48 hpf (A, B, E, F) and 4 dpf (C, D, G, H) embryos. (A, C, E, G) Lateral views, (B, D, F, H) dorsal views, rostral to the left. Vmat2-reactive neurons in ventral diencephalon can be divided into anterior group of posterior tuberculum (PTa), posterior group of posterior tuberculum (PTp) and group of paraventricular organ (asterisk), and there is a gap (white arrow) between PTa and PTp; (H) catecholaminergic clusters in ventral diencephalon of 4 dpf embryos can be divided into several groups from anterior to posterior: population 1 (between ventral thalamus and posterior tuberculum), population 2 (anterior group of posterior tuberculum), population 3 (paraventricular organ), population 4 (posterior group of posterior tuberculum) and populations 5 and 6 (between posterior tuberculum and hypothalamus); there is also a gap (white arrow) between populations 2 and 4. AAN: arch-associated neurons; DC: diencephalic catecholaminergic cluster; DV: diencephalic vmat2-expression cluster; HyV: hypothalamic vmat2-expression cluster; LC: locus coeruleus; MC: medulla catecholaminergic cluster; PTa: anterior group of the posterior tubercular neurons; PTp: posterior group of the posterior tubercular neurons; PtC: pretectal catecholaminergic cluster; PtV: pretectal vmat2-expression cluster; Ra: raphe nuclei; Sym: sympathetic neurons.
Reprinted from Developmental Biology, 314(1), Wen, L., Wei, W., Gu, W., Huang, P., Ren, X., Zhang, Z., Zhu, Z., Lin, S., and Zhang, B., Visualization of monoaminergic neurons and neurotoxicity of MPTP in live transgenic zebrafish, 84-92, Copyright (2008) with permission from Elsevier. Full text @ Dev. Biol.