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Fig. 4 In situ hybridization using a cdc25a antisense probe during epiboly. Represented are staged semi-quantitative RT-PCR results using either cdc25a (j) or ODC (k) specific primers. Cycle numbers are as indicated below panels, with stages at left. (+) plasmid positive control; (-RT) no RT control reactions. All embryos are oriented animal pole up. g and h are lateral views with the dorsal side to the right and i is a dorsal view. YSL, yolk syncytial layer; EVL, enveloping layer; n, notochord; np, neural plate. Scale bar = 20 μm.
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