ZFIN Image: Li et al., 2007, Fig. S3

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Figure Caption

Fig. S3 Cells from tfap2a/c deficient donors do not give rise to melanophores in mitf/nacre homozygous host embryos. (A) Lateral view of a 48 hpf chimera derived from a wild-type donor and a mitf/nacre homozygous host embryo nacre mutants have neural crest but lack melanophores because of a cell autonomous requirement for MITF in the melanophore lineage (Lister et al., 1999). (A′) A close-up view reveals that the melanophores found in the embryo contain biotin and thus are donor-derived (13 transplanted embryos, at least 5 donor-derived melanophores were found in each of them). (B) Lateral view of a 48 hpf chimera derived from a tfap2a^MO/c^MO donor and a mitf/nacre homozygous host embryo. Despite the presence of biotin-labeled donor-derived cells in the dorsal neural tube, no melanophores are visible in this embryo (0 melanophores were found in 18 transplanted embryos which had donor-derived cells in the dorsal neural tube). Scale bars: A, B, 100 μm; A′, 50 μm.

Acknowledgments

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Reprinted from Developmental Biology, 304(1), Li, W., and Cornell, R.A., Redundant activities of Tfap2a and Tfap2c are required for neural crest induction and development of other non-neural ectoderm derivatives in zebrafish embryos, 338-354, Copyright (2007) with permission from Elsevier. Full text @ Dev. Biol.