Fig. S2 Gene expression characteristic of neural crest is absent, but of pre-placodal region is present in tfap2a/c deficient embryos. (A, B) Dorsal views of flat-mounted, 11 hpf embryos processed to reveal sox9b expression. sox9b expression that is characteristic of premigratory neural crest (pnc) cells is absent in the tfap2aMO/cMO embryos (31 of 31 tfap2aMO/cMO embryos). (C, D) Dorsal views of flat-mounted, 11 hpf embryos processed to reveal eya1 expression. Expression of eya1 in the preplacodal region (ppr) appears normal in the tfap2aMO/cMO embryos (40 of 40 tfap2aMO/cMO embryos). Scale bars: 100 μm.
Reprinted from Developmental Biology, 304(1), Li, W., and Cornell, R.A., Redundant activities of Tfap2a and Tfap2c are required for neural crest induction and development of other non-neural ectoderm derivatives in zebrafish embryos, 338-354, Copyright (2007) with permission from Elsevier. Full text @ Dev. Biol.