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Fig. 9 plcβ3-/- neural crest cells sort from wild-type neural crest cells in mosaic embryos. (A–F) Confocal fluorescent stacks of live 6 dpf mosaic host embryos. (A, C, E) Wild-type Alexa568-labeled neural crest cells were transplanted into wild-type fli:GFP hosts at sphere stage (6 hpf). Wild-type donor cells contributed to (A) hyomandibular (hm), (C) symplectic (sy), (E) interhyal (ih), and ceratohyal (ch) cartilages. (B, D, F) plcβ3 homozygous Alexa568-labeled neural crest cells were transplanted into wild-type fli:GFP hosts at sphere stage (6 hpf). plcβ3 mutant donor cells contributed significantly to (B) hyomandibular, but not to (D) symplectic, (F) interhyal, or ceratohyal cartilages. (G, H) Schematic of cell sorting observed in mosaic transplants. (G) Wild-type to wild-type transplants did not sort, whereas (H) mutant to wild-type transplants sorted. Red indicates donor and green indicates hosts. Scale bar: 50 μm.
Reprinted from Developmental Biology, 304(1), Walker, M.B., Miller, C.T., Swartz, M.E., Eberhart, J.K., and Kimmel, C.B., phospholipase C, beta 3 is required for Endothelin1 regulation of pharyngeal arch patterning in zebrafish, 194-207, Copyright (2007) with permission from Elsevier. Full text @ Dev. Biol.