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Fig. 13 Reverse transcriptase-polymerase chain reaction (RT-PCR) analysis of sox9a (top panel) and sox9b (middle panel) in median fins of zebrafish (5.6-6.3 mm). Column 1 contains a 100-bp ladder with appropriately sized bands marked at left. Column 2, with 50 hours postfertilization (hpf) whole embryos (Chiang et al.,[2001b]), and the bottom panel, with constitutively expressed ef-1α (Nordnes et al.,[1994]), were used as positive controls. Columns 3-5 show presence and relative levels of amplified RNA for caudal fin rays, caudal hypural tissue and anal fin tissue, respectively. While sox9a is strongly expressed in the sampled median fin tissues, sox9b is not detected at all, although it is present in 50 hpf whole embryos, as expected (Chiang et al.,[2001b]). Column 6 shows RT-PCR results with no template added to the reaction mix (negative control).