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Fig. 2 Gene expression patterns of enigma variants encoding the PDZ domain. Expression was detected by whole-mount in situ hybridization in eight different developmental stages. Embryos shown are at the 4-cell stage (indicating cleavage period), 1K-cell stage around mid-blastula transition (MBT), shield stage at mid-gastrulation, and tail bud (tb) stage at end of gastrulation. All early embryos are shown in the lateral view. Furthermore, embryos at prim-6, prim-22, and long-pec stages refer to the pharyngula period and embryos at 4 days postfertilization (dpf) represent the early larval period. Lateral view of these embryos is shown in Figure 2F,H,J,K. Additionally, close-ups of frontal (E) and lateral (G) views and a dorsal view (I) of embryos at the long-pec stage are presented. am, adductus mandibulae; ba, branchial arches; cm, cephalic musculature; ce, cerebellum; hb, hindbrain; imp, intermandibularis posterior; int, intestine; ls, lens; mb, midbrain; ov, otic vesicle; pf, pectoral fin buds; som, somites.