Fig. 5 VP16-vega1 and VP16-vega2 can induce ectopic hhex expression in homozygous boz mutant embryos, and hhex expression is expanded in Dfst7 mutants. (A,B) hhex expression in a noninjected wild-type embryo. (C–F) Expression of hhex in a wild-type (C,D) and a boz mutant (E,F) embryo after injection of 100 pg VP16-vega1 mRNA. (G–J) Expression of hhex in a wild-type (G,H) and a boz (I, J) embryo after injection of 100 pg VP16-vega2 mRNA. (K,L) Expression of hhex in a noninjected embryo (K) and in an embryo injected with 25 pg of vega2 mRNA (L). (M,N) Expression of boz in a noninjected embryo (M) and in an embryo injected with 100 pg VP16-vega2 mRNA (N). (O–R) hhex expression in wild-type and heterozygous Dfst7 embryos (O,P) and in a Dfst7 homozygous mutant embryo (Q–R). Pictures are either focussed to the midline (O,Q) or the lateral marginal region (P,R). All embryos were fixed at 50% epiboly stage. All embryos depicted in A–J and O–R were genotyped after photography. (A,C,E,G,I,K–N: animal views; B,D,F,H,J,O–R: lateral views; A,B,K,M,O,P: dorsal to the right, all others, except for L,Q,R, putative dorsal to the right; probes used are indicated in the bottom left corner of each panel).
Reprinted from Developmental Biology, 276(2), Bischof, J., and Driever, W., Regulation of hhex expression in the yolk syncytial layer, the potential Nieuwkoop center homolog in zebrafish, 552-562, Copyright (2004) with permission from Elsevier. Full text @ Dev. Biol.