Fig. 3 Specification and patterning of pharyngeal endoderm is required for ectodermal expression of gcm2. (a, b). casanova is required for the expression of gcm2. gcm2 transcripts were detected by in situ hybridisation at approximately 48 hpf in wild-type, cas+/?, siblings (arrowhead in a) from a cross between known casanova heterozygotes, but staining was absent in casanova, cas-/- mutants (b). (c, d). foxi1 is required for the expression of gcm2. gcm2 transcripts were detected by in situ hybridisation at 72 hpf in wild type, foxi1+/? siblings from a cross between known heterozygotes for foxi1 (arrowhead in c), but staining was severely reduced or absent in foxi1-/- mutants (d).
Reprinted from Developmental Biology, 276(2), Hogan, B.M., Hunter, M.P., Oates, A.C., Crowhurst, M.O., Hall, N.E., Heath, J.K., Prince, V.E., and Lieschke, G.J., Zebrafish gcm2 is required for gill filament budding from pharyngeal ectoderm, 508-522, Copyright (2004) with permission from Elsevier. Full text @ Dev. Biol.