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Fig. 4 Histology of zebrafish at 4 days of development. Shown are transversal sections of zebrafish at 4 days of development stained with toluidine blue. At the one-cell stage, zebrafish embryos were either injected with a control morpholino (control) or with the cypher-MO (MO). a and b show a comparison of more anterior somites of control and MO injected embryos. Large lacunae (La) are shown within the compartments of skeletal muscle in cypher-MO injected zebrafish (b and j). Transversal sections through more caudal regions are shown in c and d, these show the more posterior somites. g and h sections show the heart, with markedly enlarged and thinned pericardium (pc) and the thinned ventricular wall (vw) in the cypher-MO injected embryos (h). g and h are not absolutely comparable in scale in order to be able to show the enlarged pericardium in h. Shown in e and f are higher magnification pictures (40×) of the ventricles of either control or MO injected embryos, respectively. The ventricular wall (vw) is marked and is thinned in the cypher-MO injected embryos (f) compared to the control (e). Higher magnification (100× oil emersion) pictures of the anterior somites shown ir MO injected embryos, respectively. The ventricular wall (vw) is marked and is thinned in the cypher-MO injected embryos (f) compared to tr MO injected embryos, respectively. The ventricular wall (vw) is marked and is thinned in the cypher-MO injected embryos (f) compared to tn a and b are shown in i and j. Large lacunae (LA) in the m
Reprinted from Developmental Biology, 299(2), van der Meer, D.L., Marques, I.J., Leito, J.T., Besser, J., Bakkers, J., Schoonheere, E., and Bagowski, C.P., Zebrafish cypher is important for somite formation and heart development, 356-372, Copyright (2006) with permission from Elsevier. Full text @ Dev. Biol.