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Fig. 2 Effects of chordin injection on ray length. (A) Regenerating fin at 3 dpa immediately before injection showing that the lengths of the regenerating rays on the dorsal and ventral sides are the same. (B) One day post injection (1 dpi) of chordin (ventral side, left side of the panel) and EGFP (dorsal side, right side of the panel) constructs at 4 dpa, a size difference between the two sides is noticeable. (C) Control fin 1 day following injection of EGFP construct in all the fin rays shows no size difference between the ventral and dorsal sides. (D-F) Pictures showing the same fin at different time points following injections at 3 dpa and 5 dpa (D: 6 dpa/1 dpi, E: 7 dpa/2 dpi and F: 8 dpa/3 dpi). A pronounced length difference of the ventral versus dorsal fin rays is observed. (G, H) Percentage length difference of dorsal EGFP-injected rays compared to ventral chordin-injected rays was calculated and plotted for rays 1-6 at 1 dpi (G) and 2 dpi (H) following a single injection of chordin (blue bars). The most noticeable differences were observed in the outer rays. A control group of fish was injected withEGFP in all dorsal and ventral rays and plotted on the same graph to show no significant difference in length between dorsal and ventral rays (red bars). Arrows indicate level of amputation. Scale bars: (A-F) = 400 μm.
Reprinted from Developmental Biology, 299(2), Smith, A., Avaron, F., Guay, D., Padhi, B.K., and Akimenko, M.A., Inhibition of BMP signaling during zebrafish fin regeneration disrupts fin growth and scleroblast differentiation and function, 438-454, Copyright (2006) with permission from Elsevier. Full text @ Dev. Biol.