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Fig. 6 Effects of morpholino-mediated knock down of Notch ligands on the differentiation of pancreatic cell populations. Panels A–D: insulin and glucagon expression at 24 hpf in control and deltaA-morphant embryos. glucagon-expressing cells are decreased in number in deltaA morphants (D). Panels E–H: glucagon expression in the pancreas (E, F) and gut (G, H) is increased in jagged1b-morphant embryos (F, H) compared with controls (E, G). Panels I–J: trypsin expression is decreased in jagged1b morphant embryos. Panels K–N: expression of endocrine and exocrine markers in jagged2-morphant embryos. insulin (red) expression is increased and trypsin (blue) expression decreased in jagged2-morphants (panels L and L′ blow up) compared with controls (panels K and K′ blow up). glucagon expression profile in jagged2-morphants is enlarged (N) reflecting the increased size of the beta-cell cluster. Embryos are in ventral view with anterior to the left. Stages are indicated at the bottom right of each frame. The scale bar is 50 μm.
Reprinted from Developmental Biology, 301(1), Zecchin, E., Filippi, A., Biemar, F., Tiso, N., Pauls, S., Ellertsdottir, E., Gnugge, L., Bortolussi, M., Driever, W., and Argenton, F., Distinct delta and jagged genes control sequential segregation of pancreatic cell types from precursor pools in zebrafish, 192-204, Copyright (2007) with permission from Elsevier. Full text @ Dev. Biol.