Fig. S1

Fig. S1 Control gli1 mismatch morpholinos have no effect on motor neuron development and expression of Hedgehog-regulated genes. Panels A, B, and C–L show dorsal and lateral views, respectively, with anterior to the left. (A and B) In a 36-hpf wild-type embryo, either uninjected (A) or control-morpholino injected (B), the islet antibody-labeled nV, nVII, and nX motor neurons are found in their characteristic positions in the hindbrain. The nX neurons are out of focus in both panels. (C and D) In a 36-hpf wild-type background, islet antibody-labeled spinal motor neurons (arrowheads) are found in similar numbers in both uninjected (C) and control morpholino-injected (D) embryos. (E–L) In uninjected 22 hpf wild-type embryos, Hedgehog-regulated genes such as net1a (E), ptc1 (G), nk2.2 (I), and gli1 (K) are expressed in characteristic patterns in the ventral hindbrain. In wild-type embryos injected with control morpholino, the expression patterns of net1a (F), ptc1 (H), nk2.2 (J), and gli1 (L) are not affected. Expression of krox20 in rhombomeres 3 and 5 was essentially the same in uninjected embryos (E, G, I) and control morpholino-injected embryos (F, H, J).