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Fig. S3 Effect of Tob1a on Formation of β-Catenin/Axin/APC Complexes. The expression plasmids were cotransfected into HEK293T cells, and co-immunoprecipitation was performed. Overexpression of Myc-tob1a had no effect on β-Catenin/Axin (A) or β-Catenin/APC binding (B). Myc-tob1a doses: 1 μg and 3 μg, respectively.
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Reprinted from Developmental Cell, 11(2), Xiong, B., Rui, Y., Zhang, M., Shi, K., Jia, S., Tian, T., Yin, K., Huang, H., Lin, S., Zhao, X., Chen, Y., Chen, Y.G., Lin, S.C., and Meng, A., Tob1 controls dorsal development of zebrafish embryos by antagonizing maternal beta-catenin transcriptional activity, 225-238, Copyright (2006) with permission from Elsevier. Full text @ Dev. Cell