Fig. 1

Fig. 1 cox1 transcripts localize to posterior mesoderm organs. Whole mount in situ hybridization of cox1 (A–H) reveals that its transcripts are ubiquitously present at shield stage (A) and localize posterior half of the embryos at tailbud stage (B–C) from side view (B) and from posterior view (C). cox1 co-localizes with pax2 (H) and fli1 (G) at 7 somite stage, indicating its expression in posterior intermediate mesoderm and posterior lateral mesoderm. During late somitogenesis stage, cox1 transcripts strongly stain posterior mesoderm (D). At 24 hpf, cox1 can be seen in various mesodermal structures (E). Expression of cox1 is not altered in casanova mutant, deficient in endoderm derivatives (F). Arrow indicates the distal end of pronephric duct, letter v indicates the vasculature and arrowhead points to the distal hypochord. Scale bar: 100 μm.