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Fig. 3 Embryonic expression of Zangptl2. Whole-mount in situ hybridization was done with a Zangptl2 probe at 18hpf (A,D), 24hpf (B,E–H) and 48hpf (C,I,J), and with a foxA1 probe at 48hpf (K). In an 18hpf embryo (A), Zangptl2 expression was detected in the yolk sac extension and spinal cord. A sagittal sections of a 18hpf embryo are shown in (D). In a 24hpf embryo (B), the expression intensity became stronger and was evident not only in the yolk sac extension and spinal cord, but in branchial arches. Higher magnification lateral (E) and dorsal–ventral views (H) of a 24hpf embryo are shown. Dotted and solid lines in (E) denote the level of sagittal sections shown in (F) and (G), respectively. In a 48hpf embryo, Zangptl2 expression was detected in the liver primordium and in pectoral fin buds (C,I,J). A 48hpf embryo stained with foxA1 known as a marker of liver primordium and gut is shown in (K). Closed arrowheads, spinal cord; closed arrows, yolk sac extension; open arrowheads, branchial arches; open arrows, pectoral fin bud; asterisk, liver primordium; double asterisk, gut. Bars indicate 50 μm.
Reprinted from Gene expression patterns : GEP, 5(5), Kubota, Y., Oike, Y., Satoh, S., Tabata, Y., Niikura, Y., Morisada, T., Akao, M., Urano, T., Ito, Y., Miyamoto, T., Watanabe, S., and Suda, T., Isolation and expression patterns of genes for three angiopoietin-like proteins, Angptl1, 2 and 6 in zebrafish, 679-685, Copyright (2005) with permission from Elsevier. Full text @ Gene Expr. Patterns