ZFIN Image: Pugacheva et al., 2006, Fig. 7

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Figure Caption

Fig. 7 Functional analysis of CTCF gene promoter region. (A) 1.4-kb genomic DNA fragment covering the 5′ flanking region (- 1300) plus a region of exon 1 (+ 84 bp) with putative conserved transcription factor binding sites. CTCF promoter constructs: promoter-less vector plasmid (pGL2), promoter region from - 146 to + 84 (pGL2 (- 146)), promoter region from - 428 to + 84 (pGL2 (- 428)), promoter region from - 995 to + 84 (pGL2 (- 995)), and promoter region from - 1300 to + 84 (pGL2 (- 1300)) are illustrated at the left. PGL2 plasmid with SV40 enhancer and promoter (pGL2 (SV40-P)) was used as positive control. Luciferase assays were repeated in three independent experiments.

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Reprinted from Gene, 375, Pugacheva, E.M., Kwon, Y.W., Hukriede, N.A., Pack, S., Flanagan, P.T., Ahn, J.C., Park, J.A., Choi, K.S., Kim, K.W., Loukinov, D., Dawid, I.B., and Lobanenkov, V.V., Cloning and characterization of zebrafish CTCF: Developmental expression patterns, regulation of the promoter region, and evolutionary aspects of gene organization, 26-36, Copyright (2006) with permission from Elsevier. Full text @ Gene