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Fig. 7 Quantitative estimation of rescue and non-rescue of the six3- and lhx2-knockdown embryos by head-specific overexpression of Lhx2 and Six3b, respectively. Rescue and non-rescue were estimated at 26 hpf based on the size of the head and the eyes in six3- and lhx2-knockdown embryos by head-specific overexpression of Lhx2 and Six3b, respectively. (A) Calculation of the relative size of the head was as follows: head thickness (ht in panel A) / whole body length (bl in panel A) x 100 (%), and the relative size of the eye was calculated as follows: diameter of the eye (de in panel A) / whole body length (bl in panel A) x 100 (%). (B) Relative size of the head (black bars) and the eye (red bars) in the embryos that were uninjected (I), six3-AMO-injected (II), six3-AMO plus caged-lhx2 mRNA-injected (without uncaging) (III), six3-AMO plus caged-lhx2 mRNA-injected (with head-uncaging) (IV) and caged-lhx2 mRNA-injected (with head-uncaging) (V). In each measurement, n = 10. (C) Relative size of the head (black bars) and the eye (red bars) in the embryos uninjected (I), lhx2-AMO-injected (II), lhx2-AMO plus caged-six3b mRNA-injected (without uncaging) (III), lhx2-AMO plus caged-six3b mRNA-injected (with head-uncaging) (IV) and caged-six3b mRNA-injected (with head-uncaging) (V). In each measurement, n = 10.
Reprinted from Developmental Biology, 287(2), Ando, H., Kobayashi, M., Tsubokawa, T., Uyemura, K., Furuta, T., and Okamoto, H., Lhx2 mediates the activity of Six3 in zebrafish forebrain growth, 456-468, Copyright (2005) with permission from Elsevier. Full text @ Dev. Biol.