Fig. 2

Fig. 2 Expression pattern of Fgf19 during zebrafish embryonic development. (A) Amplification of zebrafish Fgf19 by RT-PCR at the indicated stages. The lower panel shows results for zebrafish elongation factor 1-alpha (ef1α) as a control. (B–M) Expression pattern detected by whole mount in situ hybridization. (B) Fgf19 was expressed throughout the embryo at 14 hpf. (C–F) 20 hpf. (C) Fgf19 was expressed in the ventral telencephalon, anterior ventral thalamus, midbrain and hindbrain. (D) Dorsal view with anterior to the top. Fgf19 was expressed in the retina adjacent to the lens (arrow) and the nasal part of the lens (arrowhead). (E) Fgf19 expression in the tail bud mesenchyme (arrow). (F) Dorsal view of whole embryos, with anterior to the top. Fgf19 expression in the otic placode (arrow). (G–J) 24 hpf. (G) Fgf19 was expressed in the ventral thalamus, the ventral part of the telencephalon and the tectum and the dorsal part of the tegmentum in the brain. (H) Fgf19 expression in the otic vesicles. (I) Dorsal view with anterior to the top. Fgf19 expression was still prominent in the retina adjacent to the lens (arrow) and expanded to the entire lens (arrowhead). (J) Fgf19 expression in the somites (arrow). (K–M) 36 hpf. (K) Fgf19 was expressed in the thalamus, pretectum, midbrain, hindbrain and most posterior part of the telencephalon. (L) Fgf19 expression persisted in the lens but was no longer detected in the retina. (M) Fgf19 was expressed in the ICM. All panels show lateral views with anterior to the left and dorsal to the top, except when indicated. di, diencephalon; hb, hindbrain; mb, midbrain; te, telencephalon.