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Fig. 6 Overexpression of Crk and Crkl causes excess fusion of fast myoblasts by 26-28 hpf, and rescues splice morphants. (A-D) Examples of GFP-positive cells in embryos injected with mylz2:GFP plasmid alone (A) or together with 1.5 mM of crkl splice morpholino (B), 0.1 μg/μl crkl mRNA (C), or 0.3 μg/μl crkl mRNA and 1.5 mM crkl splice morpholino combined (D). (E) Quantitative data showing distributions of mean nuclei/fibre for individual embryos injected with crk and crkl splice morpholinos and/or crk and crkl mRNA. Scale bars: 25 μm.
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