ZFIN Image: Jasuja et al., 2006, Fig. 3

Image

Figure Caption

Fig. 3 Simultaneous knockdown of both bmp1 and mfn/tll1 expression severely dorsalizes the zebrafish embryo. Morpholinos, both targeted against 5′-UTR sequences and transcription start site, were injected at a concentration of 0.5 mM into 1–2 cell stage embryos, to a final concentration of 0.5 μM in the embryo. Embryos are shown at 12 hpf (dorsal views) (A–D) or 24 hpf (E–H) either uninjected (A and E), or injected with bmp1 (B and F) or tll1 (C and G) morpholinos, or with morpholinos to both genes (D and H). Tails are shown at 55 hpf (I–K). Arrows indicate the ruffled/notched pattern (J) and loss of ventral fin (G and K) in bmp1 and mfn/tll1 morpholino-injected embryos, respectively.

Acknowledgments

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Reprinted from Mechanisms of Development, 123(7), Jasuja, R., Voss, N., Ge, G., Hoffman, G.G., Lyman-Gingerich, J., Pelegri, F., and Greenspan, D.S., bmp1 and mini fin are functionally redundant in regulating formation of the zebrafish dorsoventral axis, 548-558, Copyright (2006) with permission from Elsevier. Full text @ Mech. Dev.