ZFIN Image: Hardison et al., 2005, Fig. 3

Image

Figure Caption

Fig. 3 The zebrafish gene claudinj is mutated in cldnj embryos. (A) Diagram illustrating the location of the proviral integration. cldnj is an intron-less gene so the integration is in the same location in the genomic sequence as the mRNA. Primer locations used in the genotyping are marked by arrowheads. (B) Genotypes of nine mutant embryos, only the mutant allele present. (C) Genotypes of nine wildtype siblings. Four wildtype and five heterozygous fish. (D) Pustell DNA matrix comparison of the sequence of EST clone FC30g01 to the published claudinj sequence. An approximately 1 kb insert in the 3′ UTR is present in the cldnj sequence but not in the EST clone.

Acknowledgments

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Reprinted from Mechanisms of Development, 122(7-8), Hardison, A.L., Lichten, L., Banerjee-Basu, S., Becker, T.S., and Burgess, S.M., The zebrafish gene claudinj is essential for normal ear function and important for the formation of the otoliths, 949-958, Copyright (2005) with permission from Elsevier. Full text @ Mech. Dev.