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Fig. 5 Nkx6 proteins regulate eng1b and chx10 expression. (A-F) Lateral views of segments 8-12. (A,B) 28-hpf embryos labeled with GABA Ab. GABA-positive cells in the VeLD and KA′ positions (denoted V-K) (see Hutchinson and Eisen, 2006) are labeled with white asterisks. nkx6 MO-injected embryos (B) have a similar number of ventral GABA-positive interneurons as control embryos (A). (C,D) 24-hpf embryos labeled with chx10 riboprobe. nkx6 double MO-injected embryos (D) have fewer chx10-positive cells than control embryos (C). (E,F) 24-hpf embryos labeled with eng1b riboprobe. nkx6 double MO-injected embryos (F) have more eng1b- positive cells than control embryos (E). (G) Quantification of GABA-positive, chx10- positive, and eng1b-positive cells in control and nkx6 double MO-injected embryos. Scale bars: 20 μm in A,B; 50 μm in C-F.