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Fig. 2 Transient transgenic expression of the gsnl1 6.4-kb reporter in zebrafish embryos. A-D: Green fluorescent protein (GFP) expression. E-H: Brightfield images. A,E: On 1 days postfertilization (dpf), GFP was detected at the nose like the endogenous gsnl1 expression, but a specific signal was not detected in the lens or the future cornea. B,C,F,G: Lateral view (B,C) and dorsal view (C,G) of 3-dpf embryos. D,H: The 5-dpf embryos. At later stages (B-D,F-H), the annular ligament and a subpopulation of the corneal epithelium were GFP-positive. The transgenic expression between 3-5 days was localized in the anterior segment of the eye, which was consistent with the endogenous gsnl1 expression pattern shown in Figure 1. al, annular ligament; ce, corneal epithelium; n, nose. Scale bar = 200 μm.