Fig. 5 Mibm132 and Mibta52b behave dominant-negatively. (A) Somite phenotype at 22ss of (a) control wt embryos, (b) mib(m132) mRNA-injected, and (c) mib(ta52b) mRNA-injected wt embryos; (d) mib(m132) mRNA-injected and (e) mib(ta52b) mRNA-injected embryos from incrosses of mibtfi91 carriers. (B) 3dpf tail and pigmentation phenotypes of (a) control wt embryos; (b) control mibtfi91 embryos; (c) type I and (c2) type II mib(m132)-injected wt embryos; (d) type I and (d′) type II mib(ta52b)-injected wt embryos; (e) type I, (e′) type II, and (e″) type III mib(m132)-injected embryos from incrosses of mibtfi91 carriers; (f) type I, (f′) type II, and (f″) type III mib(ta52b)-injected embryos from incrosses of mibtfi91 carriers. Note that type I: normal somite, curly-up tail and normal tail pigment; type II: abnormal somite, curly-up tail and normal tail pigment; and type III: abnormal somite, curly-up white tail. The numbers in the right upper corner indicate the frequency of each type (Table 3). (C) huC expression at 10ss in dorsal view of (a) control wt embryos, (b) control mibtfi91 embryos, and (c) mib(m132)-injected wt embryos; (d) mib(m132)-injected and (e) mib(ta52b)-injected embryos from incrosses of mibtfi91 carriers. (D) her4 expression at 22hpf of (a–a″) control wt embryos and (b–b″) control mibtfi91 embryos; (c–c″) mib(m132)-injected and (d–d3) mib(ta52b)-injected embryos from incrosses of mibtfi91 carriers; (a–d) head region, dorsal view; (a′–d′) trunk region and (a″–d″) tail region around cloaca, both lateral view.
Reprinted from Developmental Biology, 305(1), Zhang, C., Li, Q., Lim, C.H., Qiu, X., and Jiang, Y.J., The characterization of zebrafish antimorphic mib alleles reveals that Mib and Mind bomb-2 (Mib2) function redundantly, 14-27, Copyright (2007) with permission from Elsevier. Full text @ Dev. Biol.