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Fig. 6 No obvious structural defects in cilia in Kupffer′s vesicle or pronephric ducts of pkd2 morphant and mutant embryos. (A-D) Immunofluorescence performed with antibody against acetylated tubulin that allows for visualization of cilia. Cilia at Kupffer′s vesicle in wild-type and pkd2 augMO embryos at 12 somites (A,B), and cilia in the pronephric ducts in wild type and in cuptc321 mutants (C,D), are similar in shape and length. Wild-type Kupffer′s vesicle cilia are 4.7±1.4 μm and pkd2 augMO Kupffer′s vesicle cilia are 4.6±1.3 μm in length.
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